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dnmt1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc dnmt1
    Dnmt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+dnmt1+antibody/DNMT1+XP+Rabbit+mAb/pm41857757-52-16-20
    Average 96 stars, based on 342 article reviews
    dnmt1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Control:

    Article Title: HDAC1 Inhibition by Maspin Abrogates Epigenetic Silencing of Glutathione S-Transferase Pi (GSTp) in Prostate Carcinoma Cells
    Article Snippet: .. One aliquot was used as an input control, while the other five aliquots were used for immunoprecipitation with HDAC1 McAb, Rabbit anti-Ac-H3 (K9) antibody, rabbit anti-DNMT1 antibody, rabbit anti-DMB 2/3 antibody (Upstate Cell Signaling Solution, Lake Placid, NY), and normal rabbit immunoglobulin G mixture (Santa Cruz Biotechnology, Santa Cruz, CA), respectively. ..

    Article Title: HDAC1 Inhibition by Maspin Abrogates Epigenetic Silencing of Glutathione S-Transferase Pi in Prostate Carcinoma Cells
    Article Snippet: .. One aliquot was used as an input control, whereas the other 5 aliquots were used for immunoprecipitation with HDAC1 McAb, Rabbit anti-Ac-H3 (K9) antibody, rabbit anti-DNMT1 antibody, rabbit anti-MBD2/3 antibody (Upstate Cell Signaling Solution), and normal rabbit immunoglobulin G mixture (Santa Cruz Biotechnology), respectively. .. The resulting mixtures were gently agitated overnight at 4 C. The immunocomplexes were collected by incubation with protein G-agarose beads (Upstate Biotechnology) for 1 hour at 4 C. After the beads were washed in the sequence of low salt, high salt, LiCl and Tris-EDTA (TE) buffer, the protein–DNA cross-links were reversed by heating at 65 C for 4 hours in 3.5 mol/L NaCl, followedbyRNaseA treatment for 30minutes at 37 C and proteinase K treatment overnight.

    Immunoprecipitation:

    Article Title: HDAC1 Inhibition by Maspin Abrogates Epigenetic Silencing of Glutathione S-Transferase Pi (GSTp) in Prostate Carcinoma Cells
    Article Snippet: .. One aliquot was used as an input control, while the other five aliquots were used for immunoprecipitation with HDAC1 McAb, Rabbit anti-Ac-H3 (K9) antibody, rabbit anti-DNMT1 antibody, rabbit anti-DMB 2/3 antibody (Upstate Cell Signaling Solution, Lake Placid, NY), and normal rabbit immunoglobulin G mixture (Santa Cruz Biotechnology, Santa Cruz, CA), respectively. ..

    Article Title: HDAC1 Inhibition by Maspin Abrogates Epigenetic Silencing of Glutathione S-Transferase Pi in Prostate Carcinoma Cells
    Article Snippet: .. One aliquot was used as an input control, whereas the other 5 aliquots were used for immunoprecipitation with HDAC1 McAb, Rabbit anti-Ac-H3 (K9) antibody, rabbit anti-DNMT1 antibody, rabbit anti-MBD2/3 antibody (Upstate Cell Signaling Solution), and normal rabbit immunoglobulin G mixture (Santa Cruz Biotechnology), respectively. .. The resulting mixtures were gently agitated overnight at 4 C. The immunocomplexes were collected by incubation with protein G-agarose beads (Upstate Biotechnology) for 1 hour at 4 C. After the beads were washed in the sequence of low salt, high salt, LiCl and Tris-EDTA (TE) buffer, the protein–DNA cross-links were reversed by heating at 65 C for 4 hours in 3.5 mol/L NaCl, followedbyRNaseA treatment for 30minutes at 37 C and proteinase K treatment overnight.

    Inhibition:

    Article Title: Zebularine Significantly Sensitizes MEC1 cells to External Irradiation and Radiopharmaceutical Therapy When Administered Sequentially in Vitro
    Article Snippet: For the external-beam experiments, viability also was determined after 48 h using a WST-1 assay (Roche, USA) measuring a control of 0 Gy radiation and no zebularine, as well as 2 Gy radiation, 200 μM zebularine, and the combination of 2 Gy radiation and 200 μM zebularine. .. Inhibition of the DNMT1 target was confirmed by Western blot assay using the rabbit anti-DNMT1 antibody (Cell Signaling, Danvers, MA). ..

    Western Blot:

    Article Title: Zebularine Significantly Sensitizes MEC1 cells to External Irradiation and Radiopharmaceutical Therapy When Administered Sequentially in Vitro
    Article Snippet: For the external-beam experiments, viability also was determined after 48 h using a WST-1 assay (Roche, USA) measuring a control of 0 Gy radiation and no zebularine, as well as 2 Gy radiation, 200 μM zebularine, and the combination of 2 Gy radiation and 200 μM zebularine. .. Inhibition of the DNMT1 target was confirmed by Western blot assay using the rabbit anti-DNMT1 antibody (Cell Signaling, Danvers, MA). ..



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